Device

Part:BBa_K2277666

Designed by: Geyi Wang   Group: iGEM17_ZJUT-China   (2017-10-24)


A blue-light-sensitive self-lysis system in E.coli cells

The blue-light-sensitive self-lysis system is made by replacing the eGFP gene with lysin(part:BBa_K2277033) in part:BBa_K2277555. And the second rrnb terminator has been removed because lysin doesn't need a terminator. The system will control the E.coli cells to lyse in the absence of blue light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


[edit]
Categories
Parameters
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